PCR Cloning. Note: You clicked on an external link, which has been disabled in order to keep your shopping session open. Veuillez saisir un mot de passe. Populaire Applications et techniques Nos produits Services Assistance. Connect Your Lab Se connecter Commander.
Search All. Depending on your Ta cloning kit manual Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page. Weighing Papers and Dishes. Bioprocess Systems And Accessories. Search All. Cryogenic Storage. All reagents are guaranteed stable for 6 months when properly stored. Not for use in diagnostic procedures.
Ta iava. Specifications
Add To Cart. See Navigation. Search Kt. Why is that? Not for use in diagnostic procedures. Cat No. Search All. PCR products generated using Taq and other nonproofreading DNA polymerases can be directly cloned without any preparation. Customers who bought these products also bought Cat No. Safety Data Sheets 2. Note: You Ta cloning kit manual on an amnual link, which has been disabled in order to keep your shopping session open. Quick-Start Protocols 1. The vector kiy supplied in a linear form with a U overhang at each Ta cloning kit manual end, which hybridizes with high specificity to the A overhang of Gay guadeloupe les saintes products generated by Taq and other nonproofreading DNA polymerases. The Ligation Master Mix, supplied in a convenient premixed format, is specifically designed to provide optimal hybridization conditions for efficient cloning. These include a large number of unique restriction enzyme recognition sites, universal sequencing primer sites, and promoters for in vitro transcription.
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- Figure 1.
- Invitrogen TA Cloning technology greatly simplifies traditional restriction and ligation cloning with a one-step cloning strategy that eliminates the need for any enzymatic modifications of the PCR product and not require the use of primers that contain restriction enzyme sites.
PCR Cloning. Note: You clicked on an external link, which has been disabled in order to keep your shopping session open. Veuillez saisir un mot de passe. Populaire Applications et techniques Nos produits Services Assistance. Connect Your Lab Se connecter Commander. Search Thermo Fisher Scientific. Search All. Please purchase this product by removing it from your on-site supply center.
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For Research Use Only. Not for use in diagnostic procedures. All reagents are guaranteed stable for 6 months when properly stored. Se connecter Nouveau client? S'enregister maintenant. Fermer la page. Ajouter aux favoris.
Search Thermo Fisher Scientific. Not for use in diagnostic procedures. Search All. The T7 and Sp6 promoters of the pCR II vector allow in vitro transcription of the insert to produce sense or anti-sense products. Yes Yes No Ligation reagents included? Cat No.
Ta cloning kit manual. Customers who bought these products also bought
Don't have a profile? Catalog No. The cloning kits are available with a variety of competent cells available separately or without competent cells, depending upon your needs and budget. There is no need to add ligase and cloning is complete in 5 minutes. EcoRI sites flanking the PCR product insertion site allow for easy excision of inserts or use any combination of 15 convenient restriction sites flanking your PCR insert for easy, directional subcloning.
Just add your PCR reaction straight to the provided topoisomerase-charged vector, incubate 5 minutes, and transform E. The pCR 2. The standard in cloning When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years. Fast, simple-to-use, and efficient, TOPO cloning has been applied to many different vectors for a wide array of applications. Coli Cat. K Provide your own: for flexibility and to save money Cat.
K and K for use in isolation of clean, sequencing-ready, recombinant plasmid are also available. No offer available. View All Antibodies. Biochemicals and Reagents. Biological Buffers. Custom Services and Products.
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Sign In Don't have a profile? Please login to your online account to display your discounted pricing. Home Products Life Sciences Add to cart. Product Type. Kit, Cloning. Box 1: Topoisomerase I-activated pCR2. For Use With Application. Cloning, PCR Cloning.